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In this work, an experimental research was conducted to determine of effects hydrogen permeation a micro-alloyed heat treatment steel in order to determine the mechanical properties of the material in this work [1]. Small specimens were taken from the micro-alloyed API-X60 steel. Moreover, by the technique of the three-point test according to ASTM 399-90, the load-displacement curves for each heat treatment with and without hydrogen permeation are determined. Likewise the samples were then mechanically tested for hardness by the technique of nanoindentation to obtain the elastic modulus and hardness of the studied specimens [2]. Scanning electron microscopy (SEM) determines the type of fracture; also EDS revealed the type of precipitate formed in the surface of the material [3]. The optical analysis showed the following microstructures; ferrite/pearlite, bainite/ferrite and martensite/retained-austenite [4]. Finally the experimental and statistical results showed effect of hydrogen permeation and heat treatment on the mechanical properties of the micro-alloyed API X-60 grade pipeline steel.