The indoline al ka loids ac cu mu la tion by R. serpentina cell lines upon sur face and sub merged main te nanceThe In sti tute of Mo lec u lar Bi ol ogy and Ge net ics, NAS of Ukraine 150, Ac a de mi cian Zabolotny Str., Kyiv, 03143, Ukraine kunakh@imbg.org.ua Indoline al ka loids ac cu mu la tion, in clud ing ajmaline, in the bio mass of R. serpentina cell line cul ture on the ex am ple of hor mone in de pend ent highly pro duc tive strain K 27 at its sur face and sub merged main te nance in nu tri ent me dia with dif fer ing min eral com po si tion has been stud ied. Op ti mal nu tri ent me dia for both ways of main te nance have been iden ti fied. The con di tions for two step main te nance in sur face, fol lowed by sub merged cul tures, in compositionally sim ple nu tri ent me dia have been spec i fied. Two step main te nance in creases al ka loids ac cu mu la tion at the early stages of growth 3 4 times and al lows de creas ing the time of cal lus tis sue grow ing from 60 80 days to 20 40 days. Keywords: ajmaline, indoline al ka loids, plant tis sue cul ture, R. ser pen tine, cell lines -al ka loids producents. Primary CallusObtained by B. G. Butenko on MC medium according to [2], green body fragment of 5 year old plant, 1964 Callus Tissue MC medium, total alkaloid content 0.1-0.3 %, ajmaline 0.04 % (1964-1968) [3] Mutagen treatment by nitrogenous yperite in 1968 M1 Mutant Line MC medium, total alkaloid content 0.2-0.48 %, ajmaline app. 0.2 % [4]Selection on nutrition medium 5C, without phytohormones according to [5] (1972-1975) Cell Line A 5C medium without phytohormones, indoline alkaloid content 0.7-0.8 %, ajmaline app. 0.4 % Mutagen treatment by ethylenimine in 1981 and the adaptation to growth on 10C medium, without phytohormones according to [8] in the course of [1982][1983][1984][1985] K 27 Strain 10C medium, indoline alkaloid content 1.2-1.8 %, ajmaline 0.9-1.2 %