In a previous report optimisation of ED-XRF and TXRF for whole blood analysis has been reported. In the present work the optimised technique has been applied to investigate possible influence on pregnancy outcome due to an environment characterised by high levels of pollutants. Two groups of maternal and neonate whole blood were analysed with TXRF and GFAAS. The material of the first group was taken from Zabrze (Upper Silesia, Poland), an industrial region with a high degree of pollution. The second group consisted of controls from the Go¨teborg region in Sweden, where the levels of toxic pollutants in the environment are not as high as those in Zabrze. The results show that as compared with the controls the levels of essential elements like Zn and Se were lower in the Zabrze infants while the levels of toxic elements like Cd and Pb were higher in this group.
Background. Inhibin B (INH-B) as a marker of Sertoli cells function correlates positively with the number of these cells and negatively with the degreee of the seminiferous tubules damage. In women INH-B may serve as the marker of the ovarian reserve. In patients with congenital adrenal hyperplasia (CAH) fertility is decreased in comparison to the general population. Aim of the study. Assessment of INH-B serum concentrations in patients with CAH and evaluation of relationship between INH-B and LH, FSH, estradiol and testosterone. Material and methods. The study comprised 68 subjects (mean age: 15,0 ± 6,4 years), including 12 men, 27 boys and 13 women, 16 girls with classic CAH.Serum assays of INH-B (ELISA; OBI-DSL) and LH, FSH, estradiol and testosterone (ECLIA; Roche) were performed. Results. Mean serum INH-B concentrations in the examined group were 136,7 ± 23,9 pg/ml and in most subjects were within normal ranges for age and sexual development stage. Only in 3 men decreased serum INH-Słowa kluczowe inhibina B, wrodzony przerost nadnerczy Streszczenie Wstęp. Inhibina B (INH-B) jako marker oceniający funkcję komórek Sertoliego wykazuje pozytywną korelację z liczbą komórek Sertoliego i negatywną ze stopniem uszkodzenia kanalików nasiennych. U kobiet INH-B może służyć jako marker rezerwy jajnikowej. U chorych z wrodzonym przerostem nadnerczy (WPN) stwierdza się obniżoną płodność w porównaniu do populacji ogólnej. Dotychczas ukazało się niewiele prac na temat stężenia INH-B we krwi u chorych z WPN. Cel pracy. Ocena stężeń INB-B w surowicy krwi u chorych z WPN oraz analiza zależności pomiędzy stężeniem INH-B a stężeniem LH, FSH, estradiolu i testosteronu u tych chorych. Materiał i metody. Badania dotyczyły 68 osób (średni wiek: 15,0 ± 6,4 lat), w tym 12 mężczyzn i 27 chłopców oraz 13 kobiet i 16 dziewcząt z postacią klasyczną WPN. Wykonano oznaczenia INH-B (ELISA; OBI-DSL) oraz LH, FSH, estradiolu i testosteronu (EC-LIA; Roche) w surowicy krwi. Wyniki. Średnie stężenia INH-B w surowicy krwi w badanej grupie chorych wynosiły 136,7 ± 23,9 pg/ml i u większości badanych
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.