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synopsisThe translational and rotational dynamics of tobacco mosaic virus in sodium phosphate buffer (pH = 7.5) solutions has been investigated by polarized and depolarized light scattering Rayleigh linewidth studies. For concentrations ranging from 1.75 X 10-4 g ml-l to 0.25 X g ml-l the translational diffusion coefficient ( D T ) has been found to be slightly concentration dependent and extrapolation to zero concentration gives DO^^^^ = 0.34 f 0.01 X A full analysis of the polarized spectra obtained at high and low scattering angles and the depolarized spectra a t near zero scattering angle has enabled these techniques to be compared and the rotational diffusion constant DR to be determined. At a solution concentration of 1.75 X 10-4 g ml-1 a mean value is found to be DR~OOC = 350 f 30 s-l. These values of DT and DR are in approximate agreement with calculations based on models of the tobacco mosaic virus molecule as a cylindrical rod. cm*s-l.

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