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A brood stock of Fundulus heteroclitus (Walbaum) minnows was established at the Johnson Space Center in Houston, Texas. By use of the aquarium maintenance system described in this report the fish were induced to produce fertile eggs throughout the year. Additional data is presented to confirm that F. heteroclitus found in the vicinity of Beaufort, North Carolina produce eggs which lack chorionic fibrils, making them especially suitable for use in embryo development studies.

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Single, 1-h treatments of five stages of Fundulus heteroclitus embryos with Furanace solutions (2.0, 1.0, 0.5, and 0.25 ppm) resulted in no significant increase in either mortality or anomaly rates. We conclude that a 1-h treatment with Furanace may be safely used in the media supporting fertilized eggs. Chronic exposure of F. heteroclitus embryos in 2.0, 6.0, and 10.0 ppm solutions results in high mortality and anomaly rates.