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51%) were greater (p<0.001) than Day 6 (35%) and 7 (33%), but Day 6 euploid rates were not different from Day 7. Failed amplification rates were similar at 2%, 2%, and 3% on Day 5, 6, and 7, respectively. Euploid rates correlated with blastocyst quality on Day 5 (GOOD ¼ 55%; FAIR ¼ 47%, POOR ¼ 35%) and Day 6 (GOOD ¼ 49%; FAIR ¼ 32%, POOR ¼ 25%). but not on Day 7 (GOOD ¼ 25%; FAIR ¼ 43%, POOR ¼ 30%).Single euploid blastocyst FET pregnancy rates as detailed in Table 1 on Day 5, 6, and 7 were 75%, 63%, 35%, respectively (p<0.01). Furthermore, pregnancy rates were positively correlated to blastocyst quality.CONCLUSIONS: Although Day 7 blastocysts represented only 11% of all the blastocysts biopsied in these PGT-A cycles, the euploid rate was similar to those seen in Day 6 blastocysts, but markedly lower than Day 5 blastocysts. Furthermore, although the blastocyst quality is low in Day 7 biopsied blastocysts with only 7% GOOD quality, 35% of our patients achieved pregnancy from Day 7 single euploid FETs. These results support the continued practice of extended blastocyst culture to Day 7 to increase PGT-A pregnancy rates per retrieval cycle.

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