The AutoPBSC is a reliable system for peripheral blood HPC collection in children mainly when used in combination with LVL. The major advantage of this software is a reduced need for priming. LVL allows better CD34+ cell collection efficiency, enhanced recruitment, and improved CD34+ cell yield.

BACKGROUND: Umbilical cord blood (UCB) has become an important source of transplantable CD34 + hematopoietic progenitor cells. Cord blood banks (CBBs) can increase their efficiency by minimizing the processing of UCB units with low CD34 + content, which have a lower likelihood of transplant utilization. We sought to identify a readily available preprocessing metric that would correlate with CD34 + cell counts, without the cost of additional analysis.STUDY DESIGN AND METHODS: Data were compiled for 131 UCB units processed at the regional CBB. Preprocessing hematologic metrics, including complete blood count and differential, were compared to postprocessing CD34 + cell quantities. The data were divided into six groups of varying preprocessing metrics, then compared for significant differences in postprocessing CD34 + cell quantities to develop a screening guidance.ABBREVIATIONS: CBBs = cord blood banks; nRBC = nucleated RBC; TNC = total nucleated cell count; UCB = umbilical cord blood From the Cell Therapy Laboratory, San Diego Blood Bank, San Diego, California.