Palíková M., J. Mare‰, J. Jirásek : Characteristics of Leukocytes and Thrombocytes of Selected Sturgeon Species from Intensive Breeding. Acta Vet. Brno, 1999, 68: 259-264. Analysis of leukocyte and thrombocyte characteristics in Acipenser baerii, Acipenser stellatus and Huso huso sturgeons at the age of about 200 days kept under intensive breeding conditions is presented. The differential leukocyte count of studied fish species was of lymphocytic character (68.0-73.5% of lymphocytes), and the neutrophilic and eosinophilic granulocytes amounted to 21.8-25.1% and 3.0-4.6%, respectively. Statistically significant (p< 0.05) differences were found between individual sturgeon species in morphometric characteristics of studied cells (i.e. thrombocytes, lymphocytes, neutrophilic and eosinophilic granulocytes). The highest values were found in Acipenser baerii. These results are in good agreement with data on the ploidy in sturgeon species under study. Acipenser baerii, Acipenser stellatus, Huso huso, differential leukocyte count, thrombocytes, lymphocytes, neutrophilic granulocytes, eosinophilic granulocytesThe family Acipenseridae may be divided into two groups with respect to the number of chromosomes and the amount of nuclear DNA. The first group comprises species having a large number of chromosomes (2n = 250). The species A. baerii and A. gueldenstaedti belong to this group. The second group includes, for example, H. huso, A. ruthenus, A. stellatus with lower numbers of chromosomes in their genomes (2n = 118-120) (Vasilyev et al. 1980;Gorshkova et al. 1996;Arefjev and Nikolaev 1991). Several studies have shown correlation between the ploidy in various fish species and the red blood cell size (Arefjev and Nikolaev 1991; Flaj‰hans 1997; Svobodová et al. 1998).The aim of our study was to evaluate interspecies differences in morphological characteristics of leukocytes and thrombocytes in selected sturgeon species (i.e. A. baerii, H. huso and A. stellatus) known for different numbers of chromosomes. Materials and MethodsA total of 10 Acipenser baerii, 10 Huso huso and 5 Acipenser stellatus sturgeons originating from the Fishery Hluboká, Mydlovary farm were sampled (A. baerii in September, A. stellatus a H. huso early in December). All the fish used in the study were clinically healthy without any signs of disease. The fish were kept under intensive breeding conditions in flow-through troughs with controlled environment (water temperature of 20 o C, water oxygen saturation over 80%). Other hydrochemical indicator values assessed in discharge water characterise the environmental conditions suitable for intensive sturgeon culture. The fish were fed complete feeding mixtures. Basic haematological and biochemical parameters have been already published (Jirásek and Mare‰ 1998). Proke‰ et al. (1995ab; studied morphometric characteristics of these sturgeon species with respect to plastic and meristematic signs. Using morphometric characteristics these authors determined the studied species to be pure species.Blood fr...

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The experimental rearing of tench (Tinca tinca) juveniles of two size classes with initial individual weight of 0.8 and 1.2 g was performed into flow-through aquaria connected to recirculation system during a period of 63 days. Three feeds (KARPICO Supreme 7 Ex, ASTA 2, L05/CH) with nutrient content of 37/8, 42/7 and 35/7 (% proteins/ fat) were used in the experiment. Achieved values of production indicators – feed conversion ratio (FCR) and specific growth rate (SGR) ranged from 1.84 to 4.15 and from 0.70 to 1.49 %.d–1, respectively in relationship to the size class and feed used. More favourable values were achieved with the bigger size class regardless to the diet used (FCR 1.84–3.53 and SGR 0.83–1.49 %.d–1) and the best results were achieved when using ASTA 2 feed (2.05 and 1.84 or more precisely 1.36 and 1.49%.d–1).

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The objective of the present study was to determine the values of basic blood indicators and of biochemical plasma indicators of we Is (Si/urlls glanis L.) intensively bred in warmed power station water without natural feeds. The study included 8 feeding experiments using 4 imported (ALMA. BioMar) and 4 domestic pelle ted feeds with different protein (40 -491ft) and fat 18-22'7c) content. The experiment involved 3265 one-year-old she at fish (Su I) of 209 and 295 g average individual weight (in 1996 and 1997. respectively). Analysed were 56 fish of 15 months (1997) and 17 months (1996) Significant changes IP < 0.05) in hematological indicators were found in fish fed the PDl feed mixture and very significant (P < 0.0 I) differences in the Chol a TL values in fish fed BioMar and W 97. These data characterise the adaptation reaction of the internal environment of wels to conditions of intensive aquaculture with warmed water and the nutritional value of used feeds. Intensive aijltacliiture. Sill/rus glanis. wels. pelleted feed. blood indicawl'S.A good adaptability of wels (Silltrlts gianis L.) to various ecological, food and technological conditions enables its farming diversity in various production systems. Besides pond and combined culture, the whole wels production cycle from fry to marketable fish can be run in special facilities with controlled water temperature regime and utilizing commercial feeds (J i n1 s e k et al. 1998).The aim of the experiments performed in 1996 and 1997 was to test the production of twoyear-old wels of lower jndividual body mass in intensive aquaCUlture, The achieved production results are presented in the study of linlsek and r--lareS (1998). i\latcrials and MethodsThe experiments were carried out in the fish farm TisO\'u ICeske f) b:irst\i Mari:inske Lazne PIC). Heated powerplant effluents were used. Water temperature was controlled using the water from the Ohre river. Partly shaded concrete raceways (production volume 3 m', int10w rate 2 l·s·1 ) were utilized for the experiments. The production and physiological effects of --1 imported pelleted feed produced by ALl\IA and BioMar. one domestic pelleted feed PD 2 and 3 experimental diets of own prescriptions I indicated A. Band \V97) were tested in two replicates. The feeds differred in protein (40 -46 '7r) and lipid content (8 -22 '7(). All feeds were produced by extrusion.