Objective: The aim of the present study was to investigate in vitro antibacterial, antifungal, and antioxidant activities of different solvent extracts of Garcinia mangostana leaves. Methods:The powdered leaf was subjected to sequential extraction using hexane, ethyl acetate (EA), and methanol. The extracts were subjected to quantitative and qualitative phytochemical analysis, antimicrobial, and antioxidant activities. The best solvent extract was subjected to gas chromatography-mass spectrometry (GC-MS) analysis. Results:The highest activity was recorded in EA extract which was subjected to GC-MS analysis revealing the presence of squalene (17.09%). Conclusion:From this present study, we conclude that EA is the best solvent for extracting antimicrobial and antioxidant compounds from the leaves of G. mangostana.