Hemorrhagic fever with renal syndrome (HFRS) is endemic in East Asia and Europe. This study was initiated to investigate the reactivity of antibodies in sera of Chinese HFRS patients with the recombinant nucleocapsid proteins (rNPs) of Hantaan virus (HTNV), Dobrava-Belgrade virus (DOBV), and Puumala virus (PUUV), which are the prevalent hantavirus strains in Europe. Forty-eight pairs of acute and convalescent sera were collected from HFRS patients in Hubei, China (1985-2002) and tested by indirect IgG, IgA, and IgM enzyme-linked immunosorbent assays with six rNPs of European hantaviruses as coated antigens, respectively. The results showed that the sensitivity of rNPs against IgG was HTNV-rNP > DOBV-rNP > PUUV-rNP, while the sensitivity against IgA was DOBV-rNP > HTNV-rNP > PUUV-rNP. Quantitative analysis revealed both acute and convalescent sera from HFRS patients predominantly exhibit high levels of IgA. Although PUUV-rNPs showed very weak reactivity to the three kinds of immunoglobulins in all samples, three pairs of sera unexpectedly cross-reacted strongly to all three PUUV-rNP subtypes. We first observe that HFRS patients' sera from Hubei Province show new prevalent characteristics of cross-reacting with PUUV-rNPs and continued high level of IgA in convalescent phase, as well as in China.
SUMMARYUnder the proportional strain loading path, particle assemblies may exhibit various failure modes. Besides the strain localization, the diffuse failure may also occur under certain conditions. The diffuse failure mode corresponds to a homogeneous occurrence of failure with stress states strictly included within the plastic limit condition. This paper emphasizes the influences of the density degree and the rolling resistance under the strain path. A contact model considering rolling friction is adopted in a discrete element method analysis as an approximate means to account for the effects of particle shape. Mechanical responses indicate that loose assemblies without the rolling resistance are more vulnerable to static liquefaction. A sample with a smaller initial void ratio or larger rolling friction coefficient will reinforce the stability of the structure and reduce the likelihood of failure. For microscopic properties, the evolution of coordination numbers, contact forces, force chains and the anisotropies of the assemblies are explored and discussed. Rotational resistance helps increase the shear stress of the granular material, and the microscopic parameters indicate that the assembly has a strong anisotropy and a stable structure to resist the increasing loading.
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