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Batch cultures of Agrobacterium tumejaciens were grown in a stirred reactor holding 400 litres of a minimal medium containing 20 g of sucrose litre-1. 3-Ketosucrose accumulated in the medium during the growth phase to reach a maximum concentration of 6 8 g litre-' at the point of sucrose exhaustion, 18-20 h after inoculation. The 3-ketosucrose produced was free from other sugars. The yield of 3-ketosucrose was influenced by initial aeration rate and inoculum size.

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