This study aimed to determine the combination of plant growth regulators (PGR) that affects the growth of three provenances of Jabon merah through in vitro culture. This research was conducted in December 2017 to March 2018 at Biotechnology and Tree Breeding Laboratory, Hasanuddin University, Makassar. Research step consisted of sterilization, stock solutions and culture media preparation, and planting. The observation parameters were plant height, time of leaf formation, number of leaf, leaf length, number of root, root length, and percentage of dead explant. The results obtained from this observation were (1) the provenance significantly affected the time of leaf formation, number of root, root length, and percentage of explant explant, (2) the combination of PGR significantly affected the number of roots and root length, (3) the interaction between PGR and provenance was of a significant effect on the root length, (4) The combination of 1 ppm IBA and 0.5 ppm TDZ showed the best PGR in increasing the root length, (5) combination of 3 ppm IBA and 0.5 ppm TDZ increased the number of root, and (6) Sidrap Provenance was the best PGR based on the number of roots, root length and low mortality rate.