Objectives To evaluate the technical feasibility and anatomical and functional outcomes of laparoscopically assisted sigmoid colon vaginoplasty (LASV) in women with Mayer-Rokitansky-KusterHauser (MRKH) syndrome.Design A retrospective review of prospectively collected data.Setting Shanghai First People's Hospital, Shanghai Jiao Tong University.Population Twenty-six women with MRKH syndrome.Methods A record was made of mean operating time, length of hospital stay, perioperative complications and the anatomical and functional outcomes of surgery.Main outcome measures The perioperative results, complications and anatomical and functional outcomes of LASV (with median 20 months follow up, range 5-48 months).Results The mean operating time and hospital stay were 238 minutes and 9.8 days, respectively. The mean fall in haemoglobin was 2.0 g/dl. The only significant perioperative complications were one case with blood transfusion and three cases with infection (one with urinary tract and two with adjunctive incision). A functioning vagina 10 to 15 cm in length and 4 cm in width was created in all women. Introital stenosis occurred in only two women (2 months later). Twenty-two women subsequently had intercourse and 20 women (91%) were satisfied with the surgery and subsequent sexual activity.Conclusions LASV is an effective approach for women with MRKH syndrome. Both the anatomical and functional outcomes are satisfactory.Keywords Laparoscopy, neovagina, Rokitansky's syndrome, sigmoid transplant, vaginal agenesis.Please cite this paper as: Cai B, Zhang J, Xi X, Yan Q, Wan X. Laparoscopically assisted sigmoid colon vaginoplasty in women with Mayer-Rokitansky-KusterHauser syndrome: feasibility and short-term results.