Ultra performance liquid chromatography-photodiode array detection (UPLC-PDA) was used to analyze five biologically active polyphenolic compounds (catechin, epicatechin, rutin, trans-resveratrol, and quercetin) in different types of wine available in the Polish market. The results showed that the major polyphenols in chosen wines were catechin and epicatechin, the minor polyphenol was trans-resveratrol. The use of UPLC system allowed a shorter run time of up to six times in comparison with the conventional HPLC system. Total polyphenols were analyzed according to the Folin-Ciocalteu method using gallic acid as a standard and the results were presented as gallic acid equivalents. The wines were also analyzed for anthocyanins content using malvidin-3,5-diglucoside as a reference material in spectrophotometric analysis.