A poppy cell suspension culture was permeabilized by Tween 80 and immobilized by glutaraldehyde.The a-Galactosidase in these cells showed an optimum pH level at 5.2 and an optimum temperature at 70 "c. Enzyme hydrolysis was linear for 3 h. reaching 86% conversion. A very good level of storage stability was achieved when using dry catalyst and immobilized cells in 0.15 M NaCl solution (with the addition of chloramphenicol. [ l-methyldodecyl>dimethylamin-4-oxide (ATDNO), chlortetracycline hydrochloride (CLCTC)] or by freezing them in 0.15 M NaCl solution

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Using a synthetic substrate, a simple and sensitive procedure for the determination of extracellular lactase was developed. The enzyme studied produced by the tested plant material hydrolyzed the substrate (1‐naphtyl‐α‐D‐galactopyranoside) to α‐D‐galactose and 1‐naphthol. By simultaneous azocoupling of 1‐naphthol hardly water‐soluble azo‐dyes were produced. The evaluation of the intensity of dyed zones allowed the extracellular lactase activity to be assessed. The agar plate method described permitted rapid, simple, and specific detection of plant producers of extracellular lactase and proved to be perspectively useful in inhibitory and/or biotechnological studies.

Geum urbanum L. (Rosaceae) has been known in Europe since the 12th century (M a t h i o 1 a , 193 I), but the substances contained therein have been systematically studied only from the beginning of this century. H i r i s s e y and C h e ym o 1 (1925 a, 1925 b) and H C r i s s e y (1926) have isolated a glycoside which they