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1. Methyl-O-fl-D-fructofuranoside has been prepared by the action of yeast invertase on sucrose in the presence of methanol. 2. The fructoside has been used as a substrate for a mould-enzyme preparation (Taka-diastase). An examination of the products showed that both hydrolysis and transfructosylation occurred. 3. Sucrose could not be detected among the products of action upon mixtures of methyl ,Bfructoside and glucose, either by chromatographic fractionation or by the use of 14C-labelled glucose. 4. Taka-diastase acting on mixtures of raffinose and glucose formed sucrose. The incorporation of radioactive glucose into the molecule demonstrated that sucrose formation was the result of fructose transfer and not of melibiase (cx-galactosidase) action. 5. The implications of these findings are discussed in relation to the hypothesis that invertases form fructosyl-enzyme compounds as common intermediates in hydrolysis and transfructosylation.

SummaryMethods are described for measuring fibrinolytic activity in the rat. These include dilute blood clot lysis, euglobulin clot lysis, a fibrin plate assay with euglobulin solutions, and an accelerated whole blood clot lysis technique in which limited fibrinolysis is induced with urokinase. In addition, methods have been worked out for the estimation of four plasma components closely involved in fibrinolysis: fibrinogen, factor XIII, plasma inhibitors and fibrin degradation products. The sensitivity and validity of the assays were tested by studying their capacity to detect changes resulting from the administration of eledoisin, Neohydrin and turpentine.

When an aqueous solution of the fibroin of Bombyx mori is treated with chymotrypsin, a white precipitate is formed that contains approximately 60 % of the nitrogen of the original fibroin, and the remaining 40 % of the nitrogen remains in solution in the form of a mixture of water-soluble peptides. These two fractions, the precipitate and the mixture of soluble peptides, we propose to call fraction Cp and fraction Cs respectively, and these terms replace our earlier nomenclature CTP and CTL, which is no longer acceptable because the former has become the standard abbreviation for cytidine triphosphate. The new system has the advantage that it is applicable by analogy to the fractionation of fibroin by other enzymes: tryptic hydrolysis thus gives rise to fractions Tp and Ts, peptic hydrolysis to fEractions Pp and Ps. The precipitate, fraction Cp, is composed almost entirely of glycine, alanine, and serine, has a molecular weight of about 4000, and has a sequence that can be represented by