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Summary Observation nurseries including 24 varieties of sesame were grown at 23 locations in 16 states. Data for yield, oil and protein content of the seed, and for iodine value of the oil were obtained from certain locations. Sesame seems reasonably well adapted on the better soils of most of the southern one‐half of the United States. Average seed yield for varieties at 14 locations was 506 pounds per acre. Average yield of seed, oil, and protein per acre for seven varieties grown at eight locations was 662, 344, and 163 pounds per acre, respectively. The chemical composition of the seed was found to be well within the acceptable range but was markedly influenced both by the varieties and by the locations where they were grown. The average oil and protein contents on a moisture‐free basis were 53.53% and 26.25%, respectively. The average iodine value of the oil was 110.0. Average protein content on the moisture‐ and oil‐free basis was 57.28%. The genetic and environmental influences that affect protein content of the seed also inversely affected the oil content. It was suggested that in sesame protein synthesis is favored over oil synthesis as the nitrogen supply to the seed increases.