Due to widespread use of Sodium Dodecyl Sulfate (SDS) in households and industry followed by its subsequent disposal in waterways, there is apprehension of alarming consequences on various living organisms. In order to design effective bioremediation strategy in the tropics, efficient strains having fast degradation capacity and high optimum growth temperature are much needed. Biodegradation is the process whereby organic (i.e. carboncontaining) matter is decomposed by the action of microorganisms present in the environment. In the present investigation, a new method for isolation, selective screening optimization of SDS degrading organisms from different SDS contaminated soil. We report here the isolation of bacteria capable of SDS biodegrading by enrichment culturing from SDS contaminated soil. Totally 40 strains were isolated from enrichment media by using Basal medium. Based on the primary screening 40 bacterial strains, 4 bacterial strains were shown better zone of clearances which it taken for MBAS assay. Finally HS 18 showed an 80%, 53%, 24%, and 23% degrading activity in 1 g, 2 g, 3 g and 4 g of SDS in 100 ml respectively than other bacteria.

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Regucalcin is a multifunctional protein having an important role in calcium homeostasis, L-ascorbic acid biosynthesis, anti-prolific, anti-apoptotic functions as well as detoxification of chemical warfare nerve agents. Recently, it has been localized to male reproductive tract of rat and human, and identified as an androgen-target gene. The literature suggests a possible role of regucalcin in male fertility. However, no detailed studies have been conducted on its role in male reproductive organs of domestic animals. As an initial step, we had cloned and expressed regucalcin in Pichia pastoris. The sequence analysis showed 100% homology with regucalcin of Bos tours both at nucleotide and amino acid level. The SDS-PAGE and Western blot studies of recombinant protein probed with anti-regucalcin monoclonal antibody showed a higher molecular weight (56 kDa) than the expected (35.5 kDa) that could be due to hyperglycosylation. The recombinant regucalcin and its antibodies can be used to study the detailed role of the protein in male reproduction.