Hepatitis B immune globulin has been prepared for clinical trials in Sweden. The relative amount of antibodies to the hepatitis B antigen recovered in the respective fractionation steps was evaluated by hemagglutination, immunoelectro-osmophoresis and radial immunodiffusion. About one third of the antibody amount present in the original plasma pool was recovered in fraction II. Hepatitis B antigen was neither demonstrated in the various fractionation steps nor in the final immune globulin preparation.