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The properties of an interferon-like viral inhibitor (ILVI) released during the interaction of sensitized mouse peritoneal and/or splenic leukocytes with L-cells were studied.In addition to such properties as trypsin sensitivity, thermoresistance at 56 O C , resistance to acid pH, species specificity, cell mediated effect, etc., it has been shown that actinomycin D, puromycin and cycloheximide prevent the antiviral action of ILVI to the same extent as in the case of other interferons. The molecular weight of ILVI was found to be about 45 000. Lymphocytes seem to be the main producers of ILVI.After fractionation on Sephadex G-100 at least 2 peaks of cytotoxic activity were found in ILVI as well as in other virus-and endotoxin-stimulated interferon preparations. One of the cytotoxic components was regularly associated with the molecular species of the highest interferon activity. This finding suggests that a cytotoxic component may be involved in the antitumorigenic effect of interferon preparations.

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