Lately, land development of Jungnyeongcheon in dan-yang is accelerating further. As the impermeability layer increasing of ground, road, parking garage and so on, water quality and ecological status has been infected by non-point source. The objective of this research was look into ecological status(flora, mammals, birds, herpetofauna, fish and benthic macroinvertebrates) and water quality of Jungnyeongcheon. Findings on inquiry of ecological status, flora consists of total 101 taxa, 37 families, 77 genera, 93 species and 8 varieties. Mammals were investigated in literature survey total 5 orders, 11 families, 18 species and were verified in field survey result total 2 orders, 4 families and 5 species. Birds were investigated in literature survey total 9 orders, 25 families, 51 species and were verified in field survey result total 6 orders, 12 families, 17 species. Herpetofauna were investigated in literature survey total 4 orders, 8 families, 12 species and an amphibian were verified in field survey result total 2 families, 2 species and a reptile were verified in field survey result total 1 families, 1 species. Fish was investigated in literature survey total 4 orders, 7 families, 15 species and was verified in field survey result 101 taxa, 2 orders, 4 families, 10 species. Benthic macroinvertebrate was investigated in literature survey total 41 species, 27 families, 12 orders, 3 classes, and 3 phyla and was collected in field survey result total 22 species, 14 families, 6 orders, 3 classes, and 3 phyla. Analysis result of the water quality, BOD 5 was detected below 1.0mg/L. While T-N was detected 1.432∼3.725mg/L so that checked nonpoint pollutants inflow.