In a previous study, we reported that 13(E)-labd-13-ene-8α,15-diol (13E) possesses antiviral and anticancer activities. In this study, the anticancer and antimicrobial activities of 13(E) were evaluated against 4 cancer cell lines and 6 bacteria. 13(E) showed inhibitory effect on a variety of cancer cell lines. The IC 50 values was 8.3-21.3 µg/mL. 13(E) was the most effective growth inhibitor of murine leukaemia cell lines P388, producing approximately 8.3 µg/mL of IC 50 in the cytopathic effect (CPE) method. 13(E) also inhibited the growth of the gram-positive bacteria (Staphylococcus aureus, Bacillus cereus and Listeria monocytogenes) and gram-negative bacteria (Vibrio parahaemolyticus, Escherichia coli and Salmonella enteritidis) with a range of minimum inhibitory concentration (MIC) values from 0.092 to 0.598 mg/mL and gram-negative bacteria were more sensitive to the compound (MIC, 0.092 mg/mL).Keywords anticancer · antimicrobial · 13(E)-Labd-13-ene-8α · 15-diol Brachyglottis monroi (Hook.f.) B.Nord. (Asteraceae), previously Senecio monroi, is a shrub endemic to New Zealand (Allan, 1961;Connor and Edgar, 1987). We are not aware of any previous reports of compounds from B. monroi, but four antimicrobial diterpenes have been isolated from B. bidwillii (Bloor and Gainsford, 1993). Historically, B. repanda was traditionally used by the New Zealand Maori for treatment of sores and wounds (Riley, 1994). In our article, we reported that 13(E)-labd-13-ene-8α,15-diol (13E) isolated from B. monroi show anti-human rhinovirus 2 and 3 activities and anticancer activities on Carcinomic human alveolar basal epithelial (A549) and human larynx carcinoma (Hep2) cells (Choi et al., 2010).In this study, we have analyzed the cytotoxicity of 13(E) on 4 cancer cells (murine leukemic cells P388, murine melanoma cell line B16-F10, human cervical carcinoma cell line KB and human colon cancer cells SNU-C4) and its antimicrobial activity against three gram-positive [Staphylococcus aureus (ATCC 13565), Bacillus cereus (ATCC 10702) and Listeria monocytogenes (ATCC 15313)] and three gram-negative [Vibrio parahaemolyticus (ATCC 17802), Escherichia coli (ATCC 25922) and Salmonella enteritidis (ATCC 13076)].In our experiments, 13(E) was isolated from Brachyglottis monroi by previous report (Choi et al., 2010) and stock at -20 o C. Sulforhodamine B (SRB) and mitomycin C were purchased from Sigma-Aldrich (USA). All other chemicals were a reagent grade. The murine leukemic cells P388, murine melanoma cell line B16-F10 and human cervical carcinoma cell line KB were purchased from the American Type Culture Collection (USA). Human colon cancer SNU-C4 cells were obtained from Korean Cell Line Bank (KCLB, Korea). The KB cells were cultured in DMEM with 4.5 g of glucose/L plus 10% fetal bovine serum, L-glutamine, penicillin (50 U/mL), and streptomycin (50 µg/mL). The other 3 cells were maintained in RPMI 1640 medium (Gibco, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Gibco). Cells were grown in a humidified incubat...

We aimed to evaluate the effects of onlay-type grafted human freeze-dried corticocancellous bone block (FDBB) and deproteinized bovine bone with collagen (DBBC) loaded with Escherichia coli-produced recombinant human bone morphogenetic protein-2 (ErhBMP-2) on space maintenance and new bone formation in rat calvaria. Collagen sponge (CS), FDBB, or DBBC disks (8×4 mm) with ErhBMP-2 (2.5 μg) were implanted onto the calvaria of male Sprague-Dawley rats, whereas CS with buffer was implanted onto the calvaria as controls (n=20/carrier). Rats were killed at 2 or 8 weeks post-surgery for histologic and histomorphometric analyses; total augmented area, new bone area, and bone density were evaluated. At both time-points, all ErhBMP-2 groups showed significantly higher new bone area and bone density than the control group (p<0.05). ErhBMP-2/FDBB and ErhBMP-2/DBBC groups showed significantly higher total augmented area than ErhBMP-2/CS group (8 weeks), and ErhBMP-2/FDBB group showed significantly higher new bone area and bone density than ErhBMP-2/DBBC group (p<0.05). ErhBMP-2/CS group showed the highest bone density (p<0.05). Combining ErhBMP-2 with FDBB or DBBC could significantly improve onlay graft outcomes, by new bone formation and bone density increase. Moreover, onlaygrafted FDBB and DBBC with ErhBMP-2 could be an alternative to autogenous block onlay bone graft.