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The effect of climate and landscape characteristics on hydrologic partitioning and vegetation response is analyzed in this study. After quantifying hydrologic partitioning using the Horton index, the relation between regional climate characteristics and the Horton index is investigated. In addition, using the comparison between the predictability of the Horton index with only regional climate characteristics and the predictability of the Horton index with landscape characteristics as well as regional climate characteristics, the relative contribution of landscape characteristics on hydrologic partitioning is analyzed. Finally, investigating the predictability of the aridity index and Horton index on the normalized difference vegetation index, the effect of climate and landscape characteristics on vegetation response is estimated.