33In most species, size homeostasis appears to be exerted in late G1 phase as cells commit to 34 division, called Start in yeast and the Restriction Point in metazoans. This size threshold couples 35 cell growth to division and thereby establishes long-term size homeostasis. Our former 36 investigations have shown that hundreds of genes markedly altered cell size under homeostatic 37 growth conditions in the opportunistic yeast Candida albicans, but surprisingly only few of these 38 overlapped with size control genes in the budding yeast Saccharomyces cerevisiae. Here, we 39 investigated one of the divergent potent size regulators in C. albicans, the Myb-like HTH 40 transcription factor Dot6. Our data demonstrated that Dot6 is a negative regulator of Start and 41 also acts as a transcriptional activator of ribosome biogenesis (Ribi) genes. Genetic epistasis 42 uncovered that Dot6 interacted with the master transcriptional regulator of the G1 machinery, 43 SBF complex, but not with the Ribi and cell size regulators Sch9, Sfp1 and p38/Hog1. Dot6 was 44 required for carbon-source modulation of cell size and it is regulated at the level of nuclear 45 localization by TOR pathway. Our findings support a model where Dot6 acts as a hub that 46integrate directly growth cues via the TOR pathway to control the commitment to mitotic 47 division at G1.