The effects of inhibition of various physiological processes were evaluated with respect to flocculation of Brevibacterium sp. and it was shown that those influencing energy levels improved flocculation. Ionophores, valinomycin and gramicidin, brought about the greatest improvement in both the rate and degree. Valinomycin‐induced flocculation was influenced by the concentration of external potassium. These results demonstrate that intracellular ionic gradients exerted an influence on flocculation; dissipation of H+ or K+ gradients induced a higher level of flocculation.
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