Downloaded fromPENTOBARBITAL AND GLUCOSE UTILIZATION/Crane et al. 13permits the calculation of the rate of brain regional glucose utilization from individual animals, independent of measurements of blood flow and blood brain barrier transport. The additional measurement of brain pentobarbital levels by gas chromatography (GC) has enabled us to report the correlation between brain level of pentobarbital and the rate of glucose utilization. Methods Injection of Barbiturate and Radiolabeled 2-DOGWistar rats (200-250 g), of either sex, were injected intraperitoneally with 0.5 ml of Ringer's solution containing variable doses of sodium pentobarbital. After 10 min, the rats were placed in a restraining device (Gerling-Moore, Palo Alto) and their tails warmed for 30 sec in warm water. A 23 gauge needle was inserted into the dilated tail vein and ten fid of ( 3 H(G)) 2-DOG (sp. act., 10 Ci/mmole) in 0.25 ml of Ringers solution injected, followed by a 0.1 ml wash of Ringers solution. Two minutes later, 2 j*Ci of 2-(l-14 C)-DOG (sp. act., 54 mCi/mmole) in 0.25 ml of Ringers solution were injected through the same needle, followed by 0.1 ml of Ringers solution. Two minutes after the 14 C injection, the rats were killed by intense microwave irradiation.9 Cardiac blood was collected into a heparinized syringe for liquid scintillation counting. The time course of 2-DOG uptake and phosphorylation was determined by single ( 3 H) 2-DOG injections, followed by microwaving at varying times after injection. The brains were excised and stored at -20°C for subsequent analysis. Biochemical And Radiochemical AnalysisThe brains were thawed and the cerebral cortices were weighed and placed in Ten Broeck homogenizers with a measured amount of distilled water (=1.6 ml water -cortex wet wt X 0.8) and 50 #g of sodium secobarbital in 4 ml of methanol to serve as an internal standard in GC. Using a modified procedure of Bligh and Dyer, 10 the cortices were homogenized, 2 ml of chloroform were added, and the tissues were rehomogenized. The homogenates were centrifuged, the supernates saved, and the pellets rehomogenized in 7 ml of chloroform-methanol-water (1:2:0.8) and centrifuged. The supernates were combined, 5 ml of chloroform and 5 ml of water added and the aqueous and organic phases separated after centrifugation. The interfaces were washed three times with methanol-water (1:1), and the washes combined with the aqueous phases.The aqueous phases were further fractionated into neutral (containing glucose and 2-DOG) and anionic (containing 2-deoxy-D-glucose-6-phosphate (2-DOGP)) fractions by ion exchange chromatography on DEAE Sephadex A25 columns (1 ml bed volume). The neutral fractions were recovered unbound from the column, while the anionic fractions were eluted with 0.5M pyridine acetate, pH 5.0. Aliquots were removed from both fractions for simultaneous 3 H and 14 C liquid scintillation counting. The neutral fractions were dried by rotary evaporation and taken up in a 0.1M Tris-HCl (buffered to pH 7.5) and 0.02% sodium azide solution f...

A pilot study was conducted in the first of two monkeys using either radiolabeled Dm-Na-P or radiolabeled hydrocortisone sodium succinate, together with lidocaine HCl. This study indicated an approximately tenfold increase in the quantity of Dm-Na-P delivered to the test electrodes (4 mA; 20 minutes) whereas the quantity of hydrocortisone delivered from the test electrodes was only marginally (approximately 10%) increased as compared with that from the controls. In terms of an anti-inflammatory activity, the effective dose of Dm-Na-P in all tissue layers underlying the test electrodes was at least tenfold that of the hydrocortisone. Therefore, further trials with hydrocortisone were abandoned. In the second animal, positive test electrodes (5 mA; 20 minutes, were sited over five joints on the right side of the body and matching control electrodes (0 mA; 20 minutes) were placed over corresponding joints on the left side of the body. The control and test electrodes each contained 1.0 ml tritium-labeled Dm-Na-P (approximately 4.0 mg) and 2.0 ml 4% lidocaine HCl (80 mg). Local tissue concentrations of Dm-Na-P were higher than those that would be obtained by systematic therapy and lower than would be obtained by local injection.