The identities of two novel perennial nutsedge biotypes collected near Bakersfield, CA, were assessed using isozyme and random-amplified polymorphic deoxyribonucleic acid markers in conjunction with morphological analysis. The two biotypes, designated as CK (Cyperus rotundus cv. ‘Kempeni’) and CR (Cyperus esculentus cv. ‘Robusta’), morphologically resemble purple nutsedge and yellow nutsedge, respectively. Plants from both biotypes exhibited more prolific growth than the typical forms and possessed some traits that are not characteristic of the species they resemble. The morphological study was conducted on a total of 15 purple nutsedge, yellow nutsedge, CK, and CR populations collected in the first year and on 20 additional nutsedge populations were collected in the second year. The genetic analysis was performed on populations from the first year only. In general, there was agreement among the results obtained from the morphometric, isozymatic, and deoxyribonucleic acid studies. Populations of CR clustered with yellow nutsedge populations, indicating that CR is within the normal range of variation of this species and may represent a new introduction. Populations of CK, however, were distinct from both purple nutsedge and yellow nutsedge populations. Considering the low level of genetic variation reported in purple nutsedge and its strict vegetative mode of reproduction, CK might represent a sexually reproducing ecotype of purple nutsedge or a hybrid with yellow nutsedge.