This article deals with the assessment of methodologies for water vapor condensation determination and mold growth risk analysis on the surfaces of an organic building structure based on in-situ monitoring, carried out on the structural elements of renovated roof truss above the greenhouse “Květná zahrada” in Kroměříž. The garden including the building is signed on the UNESCO Word Heritage List for its historical and architectonical value. The monitoring was focused on hygrothermal behavior of a particular attic area during winter term 2014 - 2015. The risks of water vapor condensation and mold growth were then analyzed on the basis of data obtained by the monitoring process. These also include measurement and output data accuracy is included as well. The Results were used as recommendation for a control system of forced ventilation in the monitored attic area.