In vitro antibody generation by panning a large universal gene library with phage display was employed to generate antibodies to more than 60 different antigens. Of particular interest was a comparison of pannings on 20 different SH2 domains provided by the Structural Genomics Consortium (SGC). Streamlined methods for high throughput antibody generation developed within the 'Antibody Factory' of the German National Genome Research Network (NGFN) were demonstrated to minimise effort and provide a reliable and robust source for antibodies. For the SH2 domains, in two successive series of selections, 2668 clones were analysed, resulting in 347 primary hits in ELISA. Half of these hits were further analysed, and more than 90 different scFv antibodies to all antigens were identified. The validation of selected antibodies by cross-reactivity ELISA, western blot and on protein microarrays demonstrated the versatility of the in vitro antibody selection pipeline to generate a renewable resource of highly specific monoclonal binders in proteome scale numbers with substantially reduced effort and time.

Zusammenfassung In diesem Beitrag werden, inspiriert von praxiserprobten Book- und Paper-Sprints, unterschiedliche Optionen für ein interaktives Forschungsdatenmanagement als Vermittlungsansatz beleuchtet. An einem Schulungskonzept wird anhand unterschiedlicher Einstiege beispielhaft demonstriert, wie u. a. die aufgrund starker fachspezifischer Ausprägungen im Forschungsdatenmanagement unabdingbare Fachexpertise eingesetzt werden kann. Die mit diesem Konzept einhergehende, an vielen Stellen notwendige Flexibilität birgt vielfältige Herausforderungen, weshalb entsprechende Lösungsansätze eingehend thematisiert werden.

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