This study compared young adult men and women regarding the discrimination of musical notes following acute ingestion of alcohol. It included 20 men and 20 women aged 18 -30 years (women: M ϭ 21.95, SD ϭ 3.14; men: M ϭ 21.65, SD ϭ 2.73). This was a discrimination test of musical notes through the psychophysical forced-choice method, in which participants had to choose between two alternatives: the musical note that contained the sound frequency related to note D, F, or A, after the presentation of a standard sound frequency. The results showed significant differences between groups when comparing men and women after the ingestion of alcohol only in the D note. We found also significant differences in the comparison group of women under the influence of alcohol with each other, in the D note, and the group of men under the influence of alcohol among themselves in the F note. These results suggest that acute alcohol ingestion can change the auditory perception of men and women regarding discriminating specific musical notes (sound frequencies).