SYNOPSIS The large number of modern methods which have been published for the determination ,of total bile pigments in the blood plasma indicates the difficulties of the estimation and suggests the need for a reliable standard method. In view of the present importance of plasma bilirubin levels in the diagnosis and treatment of jaundiced subjects, six analytical procedures have been studied, each chosen as far as possible, as a representative of a group of methods each embodying different principles.A spectrophotometric method (White, Haidar, and Reinhold, 1958) and five diazo coupling procedures (Jendrassik and Grof, 1938;Powell, 1944;Perryman, Richards, and Holbrook, 1957;Stoner and Weisberg, 1957;Lathe and Ruthven, 1958) were investigated, and the results obtained by carrying out duplicate analyses by different methods on 54 stored and 64 fresh plasmas or sera are presented and discussed in this paper.

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