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Nicatinamide adenine dinucleotide (NAD) with an attached perfluoropolyether tail acts as an affinity surfactant in the extraction of the enzyme horse liver alcohol dehydrogenase (HLADH) from an aqueous medium into a fluorous solvent.

Raman spectroscopy is a powerful technique for rapid, non-invasive and reagentless analysis of materials, including biological cells and tissues. Raman Molecular Imaging combines high molecular information content Raman spectroscopy and digital full field imaging to enable the investigation of cells and tissues. We have conducted widefield imaging using a new class of birefringent liquid crystal tunable filter that provides high throughput over an extended wavelength range. This tool has been applied to investigate the linkage between reagentless spectral imaging in tissue and cells and standard reagent based approaches. In this report, we describe Raman imaging data on a clinical tissue sample and cultured cells. The results demonstrate the sensitivity of Raman Molecular Imaging and fluorescence spectral imaging to molecular differences in biological systems laying the foundation for the eventual use of this approach as a biological research and clinical diagnostic tool.