The possibility of using the enzyme (R)-Oxynitrilase in a biphasic lyotropic liquid crystal/dibutylether system has been demonstrated. This reaction system is applicable for the continuous production of (R)-benzaldehydecyanohydrin in a fixed bed reactor. The optical purity was between 94 and 96% ee and independent of the flow rate. The space time yield was maximal (2650 g/(l* d)) at a flow rate of 1.6 ml/min. KEY WORDSEnzymatic synthesis, oxynitrilase, chiral cyanohydrin, organic solvents, lyotropic liquid crystals. t To whom all correspondence should be addressed 61 Biocatal Biotransformation Downloaded from informahealthcare.com by University of Newcastle on 01/05/15 For personal use only. Biocatal Biotransformation Downloaded from informahealthcare.com by University of Newcastle on 01/05/15 For personal use only. Table 1 Kinetic constants of (R)-Oxynitrilase in citrate buffer pH = 3.75, T = 20°C and cubic liquid crystals buffer liquid crystals u,,,