Background Chronic wounds constitute a significant medical and social problem. Chronic wound treatment may be supported by various techniques, such as negative pressure therapy, phototherapy or stem cells therapy, yet most of those supporting therapies need more evidence to be used for standard wound care. Current study covers the use of sonicated Antlerogenic Stem Cells (ASC) extract on chronic wounds. Methods Study was performed on 20 dermatological patients with venous leg ulcers, divided into two groups – treated with and without ASC extract respectively. The area and circumference of the wounds during the follow-up visits were measured on the wound imprint. Dynamics of wound healing was determined and compared between control and study group; statistics includes changes in absolute values (wound area, circumference), as well as relative (percentage of wound decrease, circumference/area ratio) and their change in time. For the purpose of Ki-67 immunohistochemical staining, sections were sampled from the wound edge at distinct check-points during therapy. Results of both groups were compared with Student test or Mann-Whitney test, depending on results distribution. Results Besides Ki-67 expression, all tested wound healing parameters (including relative and absolute wound decrease and changes in circumference/area ratio) were statistically significant more favorable in experimental group. Conclusion ASC extract significantly supported standard chronic wound treatment. Due to small population of study the results should be considered preliminary, yet promising for further research.

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We developed and improved methods to extrude sediment out of core tubes and to cut defined layers of sediment in the field. For extrusion we use standard connectable 50-cm sewage tubes or aluminum rods with two lines of holes vertically in equidistance of 0.5 or 1 cm. A stopper at the bottom of the core tube is placed on top of the extruder and the core tube is pushed downwards and stopped by distance pins in the holes. The extruder is independent of the length and diameter of the core tube. A modification of the extrusion equipment can be used for checking lamination of the sediment in the field. In addition, a rubber stopper is described for the extrusion of sandy sediment out of the core tube. A PVCcore cutter is presented that is also capable of sampling watery sediments, and it prevents contamination with metal. The advantages of these devices are the simplicity, handling, robustness and cost efficiency.