The present study was conducted according to the traditional uses of Mollugo oppositifolius L. by the kabiraj (traditional practioner) for the treatment of infectious diseases of the ill fated and poor people of Bangladesh. For this antioxidant, antimicrobial and biolethality potentials were conducted by methanol (MOME), ethanol (MOEE) and petroleum ether (MOPE) extractives of the suspected species.To determine the antioxidant activity the DPPH inhibition method was used. For antimicrobial test, antibacterial and antifungal sensitivities were performed by disc diffusion method and serial tube dilution method was carried out to determine the minimum inhibitory concentrations on some human pathogenic bacteria and fungi. For cytotoxicity test, brine shrimp lethality bioassay was conducted. Among the three crude extracts, MOEE produced more significant inhibition of DPPH (IC 50 ; 27 μg/ml); MOPE produced highest zone of inhibition against Bacillus subtilis (16.67 mm) and Microsporum spp. (16.0 mm). On the other hand, MOME produced mild cytotoxicity as 50% and 90% mortality (LC 50 and LC 90 ) 8.0 μg/ml and 85.12 μg/ml. ExtractionThe plant was subjected for shade dry. [7] The dried plant was then ground into coarse powder and subjected for hot extraction [8] with methanol, ethanol and petroleum ether by Soxhlet Apparatus (Quickfit, England). [9] Each extraction was carried out about 18 h and the extracts How to cite this article: