3D printing is a growing processing technology, which offers manufacturing of tailored, portable and integrable electrochemical energy harvesting device for realising next generation bioelectronics devices. Enzymatic biofuel cells (EBFCs) associated with biocatalysis uses biofriendly alternatives for energy harvesting. Also at microscale, the precision design and assembling of the bioelectrodes are complex procedures. The combination of computer-assisted design and 3D printing has enabled the realization of customized electrochemical miniaturized devices for various applications. In this work, a completely 3D printed EBFC at a micro level configuration, names as 3D-µEBFC, integrated with new precise bioelectrode configuration has been demonstrated. The 3D-µEBFC consists of bioelectrode with comb-like structures with carbon black which helps in increasing the active surface to volume ratio available for electrocatalysis by 80 times higher than plain electrodes. This micro-device produced an output power density of 13 µW/cm2 with an open circuit voltage of 570 mV. The 3D printed bioelectrodes show high stability, which may transform the fabrication methodology by decreasing production costs and time, letting the development of complex-shaped and purely 3D printed micro-devices.