Cell suspension cultures of Camellia sinensis were established in 250 ml shake flasks. Flasks contained 50 ml liquid medium of either Murashige and Skoog (MS), N/5 MS or Heller medium containing different levels of 6-benzyladenine (BA) (0.05-2 mg l -1 ), 2,4-dichlorophenoxyacetic acid (2,4-D) (1-10 mg l -1 ), and sucrose (10-50 g l -1 ). Moreover, the pH of the medium was varied from 5.2-6.2. In addition, cultures were subjected to light irradiation as well as to complete darkness. Following optimization of aroma and terpenoid extraction methods, cell cultures were analyzed for the volatile compounds using GC/MS. A total of 43 compounds were identified using the micro SDE apparatus. Among the major monoterpenoids obtained were a-terpineol and nerol. Moreover, other high aroma-value compounds, including 2-ethyl hexanol, benzyl alcohol, benzene acetaldehyde, nonanal and phenylethylalcohol were also detected. The highest levels of these compounds were obtained from cell suspension cultures grown in MS medium containing 5 mg l -1 2,4-D, 1 mg l -1 BA and 30 g l -1 sucrose at pH of 5.8 with incubation in complete darkness.