Background: In Cameroon, C. trachomatis screening is not routinely practiced, and its epidemiology is still unexplored. The present study aimed to determine the prevalence of C. trachomatis infection, its risk factors and the genotypes circulating in the West Cameroon region.Methods: A cross-sectional study was carried out amongst patients in five district hospitals in the West region of Cameroon. Endocervical samples were collected from women visiting the hospitals forantenatal, prenuptial and contraception consultations and at least 18 years old, sexually active, and non-menstruating. The molecular detection of C. trachomatis was performed using conventional polymerase chain reaction (PCR) followed by sequencing of the ompA gene.Results: The prevalence of C. trachomatis infection was determined to be 11.47%. Having sex for the first time between the ages of 15 and 17 (OR=1.683, 95% CI: 1.1-2.5), non-usage of condom (OR=1.622, 95% CI: 1.2-2.1), being single (OR=1.263, 95% CI: 1.0-1.5) and age range 18-30 years (OR=1.426, 95% CI: 1.1-1.8) were risk factors for C. trachomatis infection. Three genotypes of C. trachomatis circulated in West Cameroon viz. D (49%), E (29.4%) and G (21.6%).Conclusions: This study revealed that, three genotypes; D (dominant), E and G were identified circulating in the population of the study area. This information may be important for controlling the dissemination of C. trachomatis infection in West Cameroon as well as strategizing the therapeutic approach.