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The antifungal activity of 38 Polyporaceae sensu lato against yeasts and dermatophytes was tested in vitro by the agar dilution method. Strains were typed organisms and clinical isolates. In this first report, Pycnoporellus fulgens (Fr.) Donk was found to be the most active species against pathological fungi and showed broad-spectrum antifungal activity against yeasts (Candida albicans, Candida glabrata) and dermatophytes (Trichophyton mentagrophytes, Trichophyton rubrum, Microsporum canis, Microsporum gypseum, Epidermophyton floccosum). Other Polyporaceae species also showed antifungal activity, but only against dermatophytes.