The present study was aimed to screen the anti-bacterial properties of medicinally important ferns viz., Adiantum caudatum L., Adiantum latifolium Lam. and Adiantum lunulatum Burm against the selected UTI pathogens. Anti-bacterial study was carried out by disc diffusion method against the pathogens viz., Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus maximum degree of antibacterial activity was observed in A. latifolium followed by A. caudatum whereas A. lunulatum showed comparatively less degree of antibacterial activity. Further work will accentuate the isolation and characterization of active principles responsible for bio-efficacy.