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ABSTRACT The aim of the present study was to evaluate the effect of biostimulant containing amino acids and yeast extract on the physiological and reproductive characteristics of mango cv. Tommy Atkins during the shoot maturation phase in tropical semi-arid region. The experimental design consisted of randomized blocks with five treatments, five replications and five plants per plot. Treatments consisted of: T1) two foliar sprays with [biostimulant + KCl] + two foliar sprays with K2SO4; T2) No biostimulant and four foliar sprays with K2SO4; T3) three individual foliar sprays with biostimulant and one foliar spray with K2SO4; T4) two foliar sprays with biostimulant and two foliar sprays with K2SO4; and T5) two foliar sprays with [biostimulant + K2SO4] + one foliar spray with K2SO4. There is a positive effect of the biostimulant containing amino acids and yeast extract on transpiration, internal CO2 concentration, water-use efficiency and number of reproductive and non-differentiated shoots of mango cv. Tommy Atkins cultivated under tropical semi-arid condition, with attenuating effect on plant abiotic stress. For shoot maturation of mango cv. Tommy Atkins, three foliar sprays with biostimulant containing amino acids and yeast extract (10 mL per plant) and one with K2SO4 (3%), starting at 45 days after paclobutrazol application (T3), can be recommended.