The purpose of this study was to investigate the difference between organizational structure types, and management levels, in terms of perceived levels of knowledge management practices within organizations. Data were collected from a sample of 155 individuals employed in San Diego County by way of a web-based survey, and were analyzed using analysis of variance (ANOVA). The results of the study indicate that the knowledge management practices of knowledge transfer, information filtering, and knowledge culture are influenced by organizational structure type. According to the study, knowledge sharing training is not significantly influenced by organizational structure type. Furthermore, the results of the study indicate there is no significant influence of management level on the knowledge management practices of knowledge transfer, information filtering, knowledge-sharing training, and knowledge culture. In summary, the results of this study solidify the assertion that organizational structure types do influence knowledge management practices.