A novel experiment has been developed with a goal of enhancing engagement and deepening understanding of fundamental molecular biology concepts. In this laboratory activity, students investigate the composition of pet foods through quantitative polymerase chain reaction (qPCR) analysis. By conducting this experiment, students address a scientific question that has the potential to be of personal interest and they gain an appreciation of the power of genetic analysis. To develop this activity, ingredient lists of popular pet foods were surveyed and eight different components were selected to be the subject of inquiry. Reaction conditions for qPCR were validated and optimized, and a simple, efficient, and student‐friendly protocol was developed. A cohort of upper‐level biology students extracted DNA from pet foods and conducted the qPCR reactions. Specificity of amplification was determined by conducting melting point analysis and agarose gel electrophoresis of the products. Student reactions were analyzed and efficacy of this approach for deepening understanding of fundamental biochemical and molecular biology concepts was documented. © 2018 International Union of Biochemistry and Molecular Biology, 46(5):536–546, 2018.

BackgroundThe breed-defining dorsal ridge in Rhodesian Ridgeback dogs is the result of a 133,000 base pair duplication on chromosome 18. Because this trait is dominant, heterozygous dogs cannot be discriminated from those with two copies of the Ridge allele.ResultsA quantitative PCR test was developed and dogs of known genotype were used as test subjects. In all cases, the correct genotype was determined experimentally.ConclusionsThis work provides a rapid and accurate methodology for determining dog genotype with respect to the Ridge allele.

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EvoS is a consortium of evolutionary studies programs that can catalyze evolutionary training across the curriculum in higher education. This special issue of Evolution: Education and Outreach shows how the dictum "Nothing in biology makes sense except in the light of evolution" can be achieved within the biological sciences and expanded to include all human-related subjects, both for undergraduate education and faculty interactions, fulfilling the ideal of a liberal arts education and turning the university into a single intellectual community.

The peer-reviewed and psychometrically validated Test of Scientific Literacy Skills developed by Gormally et al. was used to assess the strengths and weaknesses of a general education natural science program. By comparing the scores of students who had already taken at least one course in this area with the scores of those who had not, and by relating scores to students' perceptions of learning about science, a richer understanding of our program has emerged. The ability to focus on particular skill sets and areas of proficiency has allowed us to identify specific areas to target for improvement.