[Purpose] The purpose of this study is to examine the effect of calf muscle massage on ankle flexibility and balance. [Subjects and Methods] The study’s subjects were 32 healthy college students, divided into two groups according to the massage methods applied. Both groups received five-minutes of massage to each calf, making a total of ten minutes. Massage group A received effleurage, tapotement and pressure, and massage group B received effleurage, friction and petrissage. The functional reaching test and the modified one leg standing test were performed to measure the flexibility and balance of the ankles both before and after the massage application. [Results] In the functional reaching test performed after the calf muscle massage intervention, both groups showed significant increases. However, no significant differences were found between the two groups. In the modified one leg standing test, massage group A showed a significant increase in flexibility and balance after the intervention only when the left foot was used for support. Massage group B showed a significant increase when each foot was used for support. A significant difference between the groups was only observed only when the left foot was used for support. [Conclusion] Calf muscle massage may be effective for enhancing the flexibility and balance function of the ankle joint. In particular, a combined application of friction and petrissage massage techniques may be more effective for increasing ankle joint flexibility. Therefore, the application of calf massage can be considered an appropriate method for improving balancing ability.