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We were unable to detect a significant effect of donor age, up to 90 years, and preoperative donor ECD, above the lower limit of 2200 cells/mm, on endothelial failure at 5 years following PK.

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Purpose Confocal microscopy can give images of high magnification and resolution in undisturbed living tissue. It provides new information about the cellular structure of the cornea. Our aim was to measure the density, size and distribution of keratocytes.Methods Healthy cornea in four subjects was examined using tandem scanning confocal microscopy. Methods for digital analysis of images were developed.Results Keratocyte density in confocal cross sections was greatest immediately under

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