The total and trichloroacetic acid soluble fractions of glycogen were determined in various portions of the rat heart. The amount in each portion was compared on the basis of the nitrogen content of the same sample. The total glycogen concentration was highest in the atria, lowest in the left ventricle and intermediate in the septum and right ventricle. A similar distribution was found for TCA soluble glycogen. The possible role of glycogen in cardiac energetics and structure is discussed.

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