AbstrakLactobacillus bulgaricus menghasilkan asam laktat dan bakteriosin yang memiliki efek farmakologik, di antaranya sebagai antibakteri. Klebsiella pneumoniae penyebab pneumonia masih merupakan masalah kesehatan masyarakat di negara tropis.

This research was conducted to know the effectiveness of filtrate bacteriocin Lactobacillus acidophilusaccording to concentrations toward zone of growth inhibition contaminant bacteria from swordfish(Auxis rochei)stew. This research was an experimentally research conducted in laboratory of Food Chemical Research Center of LIPI Bandung used completely randomized design with two replications. Species of contaminant bacteria used wereBacillus sp. (RR2) (Da, Te, Amp, Sxt), Staphylococcus aureus(R4) and Staphylococcus aureus (R5) (Da) witha range of concentration the filtrate bacteriocin culture L.acidophilus50, 60, 70, 80, 90 and 100%. The parameters were diameter (mm) of the zones of bacterial growth inhibition.The Results showed that treatment with filtrate of bacteriocin concentration 50 to 100% effective can inhibition the growth of bacterial contaminants. This treatment privide that the filtrate bacteriocin of culture L.acidophilus can be used as bio-preservativein the swordfish stew.

ABSTRAKProtease alkaline and keratinase are a group of protease enzym which have important value in detergen industry and skin tannery. Brevibacillus agri A-03 is thermophilic bacteria isolate that comes from Ambayan Sumatera Barat hot spring and has the ability to produce protease and keratinase. The purpose of this research is to get protease alkaline and thermostable keratinase from Brevibacilus agri-A03. thermostable enzym is produced from enzym production enzym that contains kasein and keratin at various medium pH, inoculum incubation temperature and medium type. Enzym activity is measured by modified Walker methode, protein content is measured by Lowry methode. Protease alkaline is produced at exponential phase, maximum at 18 th hours of incubation and keratinase is produced at stationer phase, maximum at 22 nd hours. Both enzym is produced optimically at medium pH condition 9.0; incubation temperature 55°C, inoculum 5% by using modified Johnvesly and Naik medium with each protease and keratinase specific activity 1.927 and 1.047 U/mg

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