The current study aims to evaluate the impact of yoga therapy on hormonal imbalance in PCOS affected women. An empirical study was conducted on 25 women having been diagnosed with PCOS condition.  The study used pre and post design.  Before starting allopathic medication participants were explained about yoga therapy and participants in the study voluntarily agreed to participate in this study without using medication.  Written consent was obtained from the participants. Testosterone, Prolactin, Luteinizing Hormone (LH), Follicle Stimulating Hormone (FSH), were selected as dependent variables for this study. Before introducing the yoga therapy intervention, baseline hormonal values were collected through blood tests. All the 25 participants practiced yoga protocol specially designed for PCOS condition, for a duration of 12 weeks.  At the end of 12 weeks the participant took the blood test in the laboratory for hormonal levels of PCOS related hormones selected for this study.  Baseline data was compared with Post data.  The impact of yoga therapy on the hormonal imbalance was statistically analysed through paired t-test. The statistical analysis yielded following t values: Serum Testosterone 21.52 (P <0.001), Serum Prolactin 22.13 (P<0.001), Serum LH 30.10 (P<0.001), and Serum FSH 14.16 (P<0.001). The impact of yoga therapy intervention on hormones related to PCOS condition was found to be significantly positive. The results imply that regular yoga practice can be an effective therapy for women with PCOS, especially for correcting imbalance in hormone levels related to PCOS.