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Fixation CO 2 with microalgae is a potential promising technique to reduce CO 2 emission for its various advantages. In this work, we develop a microalgal fixation CO 2 system to capture CO 2 from actual flue gas. At first, we evolved a strain Scenedesmus obliquus, named S. obliquus WUST4, with a UV mutagenesis technique, which can endure actual flue gas. The S. obliquus WUST4 can tolerate high concentration CO 2 , and possesses high CO 2 fixation ability. Then, we developed a pilot scale microalgal fixation CO 2 system to capture CO 2 from actual flue gas with the evolved S. obliquus WUST4. The flue gas was discharged from the combustion chamber in a coke oven. A 100 L air-lift photobioreactor was built in this system. In the pilot scale system, CO 2 can be captured from the actual flue gas by the S. obliquus WUST4. The CO 2 removal ratio can reach 67% with the optimal operation conditions. This provides an efficient technique for CO 2 capture. ' EXPERIMENTAL SECTIONMicroalgae Strain and Culture Conditions. S. obliquus was used in this work. It was obtained from Institute of Hydrobiology

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Waste paper was studied as a potential source for lipid production using the oleaginous yeast Cryptococcus curvatus for the first time. Three common types of waste paper, office paper, newspaper, and cardboard, were directly hydrolyzed by an enzyme cocktail to generate sugar-rich and nitrogen-limited hydrolysates. When these hydrolysates were used without any auxiliary nutrients by C. curvatus, the lipid content and lipid yield were higher than 50% and 200 mg/g, respectively. The nitrogen-rich enzyme cocktail exerted no negative effects on lipid production. Moreover, the integrated processes of enzymatic hydrolysis and lipid fermentation achieved comparable lipid yield to the separate hydrolysis and lipid production process. The resulting lipid samples had similar fatty acid compositional profiles to those of vegetable oils, which suggested their potential for biodiesel production. These findings strongly supported waste paper as appealing substrates for lipid production via oleaginous yeast, which provided cost-effective waste paper-to-lipids routes for sustainable biodiesel production.