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Purpose To investigate the synergistic effect of Vitamin D and neferine on colorectal cancer (CRC) growth and metastasis. Methods First, the biologically active form of vitamin D, VD3 was conducted to investigate significant synergistic effect with neferine on CRC treatment by bliss analysis. Next, the colony formation and wound healing ability, the migration and invasion ability, the epithelial mesenchymal transition of HCT-116 cells under combination treatment of VD3 and neferine were assessed. Results VD3 and neferine showed synergistic effect on CRC cell growth both in a relatively low dose. The wound healing and colony formation ability, cell migration and invasion abilities were all decreased by combination use of VD3 and neferine, in comparing with VD3 or Neferine single treated group. Moreover, VD3 and Neferine significantly decreased N-cadherin, vimentin, Snail and Slug expressions in HCT-116 cells. Conclusion These data suggest that neferine enhances the anticancer capability of VD3 and reduces VD3 dose dependency. Combination of Vitamin D with neferine seems to be a potential therapeutic strategy for CRC.

Purpose To investigate the synergistic effect of vitamin D and neferine on the growth and metastasis of colorectal cancer (CRC). Methods The synergistic effect of biologically active form of vitamin D, VD3 and neferine on the treatment of CRC was investigated by bliss analysis. Colony formation and wound healing ability, migration and invasion ability, and epithelial mesenchymal transition of HCT-116 cells, as a response to the combination treatment with VD3 and neferine were evaluated. Results VD3 and neferine showed a synergistic effect on CRC cell growth at a relatively low dose. The wound healing and colony formation capacity, cell migration and invasion abilities were all decreased by combination use of VD3 and neferine, compared to the VD3 or neferine treated single group. Furthermore, VD3 and neferine significantly decreased the expressions of N-cadherin, vimentin, snail, and slug in HCT-116 cells. Conclusion These data suggest that neferine enhances the anticancer capability of VD3 and reduces the dose dependency of VD3. The combination of vitamin D with neferine appears to be a potential therapeutic strategy for CRC.